Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) which contain histone peptides with various modifications probed with Cat. No ABE1015, Anti-monomethyl-Histone H3 (Lys79) at a 1:1,000 dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Chromatin Immunoprecipitation Sequence Analysis: A representative lot used by an independent laboratory detected Histone H3 (Lys79) in NCCIT cell lysate (Jung, I., et al. (2012). Genome Res. 22(6):1026-1035).
Western Blotting Analysis: A representative lot used by an independent laboratory detected Histone H3 (Lys79) in yeast whole cell extracts (Oh. S, et al. (2010). Biochem Biophys Res Common. 399(4):512-517).

Detect Histone H3 using this rabbit polyclonal antibody, Anti-monomethyl-Histone H3 (Lys79) Antibody validated for use in western blotting, Dot Blot & ChIP-seq.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Linear peptide corresponding to human Histone H3 (Lys79).

Physical form

Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in Hela/AE cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Histone H3 (Lys79) in 10 µg of Hela/AE cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~ 17 kDa observed